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ExoView R100

Winner of the SLAS 2019 New Product Award!

A step forward in characterization capabilities within the extracellular vesicles (EV) field. The fully automated platform provides multi-level and comprehensive EV measurements for particle size analysis, EV count, EV phenotype, and biomarker colocalization. The ExoView™ platform provides previously unattainable information in a single and bias free sample workflow.

ExoView™ works without the need for sample purification, and brings the researcher one step closer to analyzing a sample in its natural state. ExoView™ R100 is an affinity based technology that allows specific populations of EVs to bind in a multiplexed manner to a functional ExoView™ chip. The fully automated instrument can measure 9 samples automatically, direct from sample, saving cost, time, and reducing purification biases.

 

For Research Use Only. Not for use in diagnostic procedures.

For Research Use Only. Not for use in diagnostic procedures.

 

ExoView™ R100 Key Features:

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multicolor fluorescence

Automated 3 color fluorescence imaging across the visible spectrum

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EV SIZE

High-resolution sizing of single EVs

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EV COUNT

Single EV counting based on affinity

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BIOMARKER COLOCALIZATION

Measure EV sub-populations through relative protein expression of multiple markers

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MULTIPLEXED SAMPLE ANALYSIS

Capture and characterize EV sub-populations from a single sample against up to 6 surface proteins

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PURIFICATION NOT REQUIRED

Bypass purification biases with single particle counting

Streamlined workflow

Automated instrument measurement increases sample throughput

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EV CARGO

Probe for cargo in single EVs

 

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Measure up to 4 markers on single EVs:

The ExoView™ platform provides the ability to measure up to 4 markers on single EVs. The multiplexed array allows up to 6 markers to be probed in parallel through binding of the EVs to an ExoView™ chip. The addition of fluorescent antibodies provides the ability to probe for an additional 3 markers with single fluorescent antibody sensitivity.

The image shows the binding of single EVs to a CD9 antibody spot, interferometric imaging measurements are shown to the left and 3 color fluorescence on the right. A heat map of marker expression on single EVs can be generated (external or luminal proteins), while simultaneously measuring their size and count.

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Specifications:

 

Minimum Particle Size Scatter

50nm

 

 

5x105 to 1x108 particles/mL

Concentration Linearity


Better than 10 fluorescein equivalents

Fluorescence Sensitivity


410nm (interferometry)
488nm (fluoresence)
555nm (fluoresence)
640nm (fluoresence)

Excitation Wavelengths

 
 

For Research Use Only. Not for use in diagnostic procedures.

ExoView™ Kits

ExoView™ Kits contain single use microarray chips for use with the ExoView™ R100 imaging platform. Each kit contains 16 chips for ultrasensitive and specific capture, and characterization of extracellular vesicle populations. 35μL of unpurified plasma or conditioned media is added directly to the ExoView™ chip, removing the biases associated with sample purification. EVs expressing specific surface markers will bind to specific antibody spots printed directly onto the microarray chip where they can be characterized further.


ExoView™ Kit Key Features

 
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MULTIPLEXED SAMPLE ANALYSIS

Capture can characterize multiple populations of EVs from a single sample using many surface markers

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COLOCALIZATION

Measure EV sub-populations through relative protein expression of multiple markers

 
 
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PURIFICATION NOT REQUIRED

Bypass purification biases with single particle counting

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STREAMLINED WORKFLOW 

Automated instrument measurement increases sample throughput

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VALIDATED ASSAYS

Range of validated capture antibodies available.  Custom assays are also available 


 
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  • Tested using cell culture media, plasma, serum, urine, CSF, salvia, and others. Purification is not required.

  • Sample volume: 35μL or 250μL

  • 1 Sample per chip, multiple samples can be incubated and measured in parallel

  • Custom chips available

  • Kits supplied with all reagents required for ExoView assay

  • Fluorescent antibodies (CD9/CD63/CD81) supplied to probe for biomarker colocalization on single EVs


ExoView™ Kit Configurations

 

 
  • CD9

  • CD63

  • CD81

  • Mouse IgG Negative Control

ExoView™ Tetraspanin Kits

 

 
 
  • CD9

  • CD63

  • CD81

  • CD41a

  • Mouse IgG Negative Control

ExoView™ Tetraspanin PLASMA Kits

 

 
 

ExoView™ tetraspanin custom kits

  • CD9

  • CD63

  • CD81

  • Up to 3 additional customer supplied capture antibodies

  • Mouse IgG Negative Control

 
 

 
 
  • Up to 6 customer supplied capture antibodies

  • Mouse IgG Negative Control

ExoView™ custom kits

 
 

ExoView™ Kit Workflow

 
 

For Research Use Only. Not for use in diagnostic procedures.