BIOMARKER COLOCALIZATION THROUGH FLUORESCENCE

The ExoView™ platform provides the ability to measure up to 4 markers on a single extracellular vesicle, with single binding event sensitivities. Measure even the smallest EVs with confidence.

 
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Key Features of Fluorescent Measurements

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FLUORESCENCE

3 color fluorescence. Single binding event sensitivities mean that even the smallest EVs are detectable

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BIOMARKER COLOCALIZATION

Measure up to 4 proteins per EV. Measure EV sub-populations through relative protein expression

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EV CARGO

Probe for luminal EV proteins and cargo in single EVs

 
 

3 COLOR FLUORESCENCE

Measure 3 color fluorescence on single EVs which are bound to the ExoView™ microarray chip.

Extracellular vesicles are captured from solution without purification and bound to one of 6 different capture antibodies (plus control) on the surface of the ExoView™ chip.

Secondary fluorescent antibodies can be added, and up to 3 colors can be measured simultaneously. The secondary antibodies are common to all 6 capture antibodies.

Single binding event sensitivities mean that even poorly expressed proteins on the smallest of extracellular vesicles are detectable through the high sensitivity of ExoView™.

 

 

BIOMARKER COLOCALIZATION

Biomarker colocalization (4 markers per EV) allows unique sub-populations of extracellular vesicles to be identified and measured.

This image shows vesicles bound to a single anti-CD41a spot on the ExoView™ chip. All vesicles imaged on this spot are therefore positive for CD41a.

Once bound, the vesicles have been stained with a 3 color fluorescent cocktail of antibodies against CD63/CD81/CD9. 

Blue Spots are vesicles that are positive for CD41a + CD63. 
Green Spots are vesicles that positive for CD41a + CD81. 
Red Spots are vesicles that are positive for CD41a + CD9. 
Other colors are vesicles that express various combinations of R/G/B fluorescent signal and CD41a (via capture on surface). 

All vesicles are simultaneously sized and counted.

 

 

DETECTING EV CARGO THROUGH FLUORESCENCE

Once bound to the ExoView™ chip, extracellular vesicles can be permeated and probed for luminal proteins and cargo. 3 color fluorescence allows a combination of staining of 3 surface and/or luminal proteins on single extracellular vesicles.

These experiments show the ability to detect luminal proteins such as ALIX and Sytenin through the permeabilization of bound extracellular vesicles.

 
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Measure up to 4 markers on a single extracellular vesicles